Study on intermittent hypoxia in children sleep apnea hypopnea syndrome model and insulin-like growth factor-1 and insulin-like growth factor binding protein-3 levels in serum
10.3760/cma.j.issn.1673-0860.2012.03.009
- VernacularTitle:间歇缺氧对幼龄大鼠血清中胰岛素样生长因子水平的影响
- Author:
Jin HOU
1
;
Jing YAN
;
Quan-Qing KANG
Author Information
1. 西安交通大学医学院第二附属医院
- Keywords:
Anoxia;
Insulin-like growth factor I;
Insulin-like growth factor binding protein 3;
Sleep apnea syndromes;
Didease models,animal;
Child
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(3):218-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using rats fed in intermittent hypoxia environment to study the relationship between sleep apnea hypopnea syndrone (SAHS) of children and growth retardation. Methods The hypoxic chamber was designed and manufactured,the control of intermittent hypoxia was achieved.Twentyfour rats were randomly divided into three groups:mild and severe hypoxia group,and control group. In control group,the animals were normally fed,without interruption.The animals in other two groups were kept in the cabin,simulated mild and severe intermittent hypoxia conditions 8-hour a day,a total of 35 days.According to the results of preliminary experiments,the concentration of intermittent hypoxia and frequency were determined. The animals with mild hypoxia events occurred nearly six times per hour,the average minimum oxygen saturation dropped to 0.853,the animals with severe hypoxia events occurred nearly 24 times per hour,the average minimum oxygen saturation dropped to O.776. Body mass and length were measured before and after experiment.The serum insulin-like growth factor (IGF)-1 and insulin-like growth factor binding protein (IGFBP)-3 expression were tested from venous blood by enzyme-linked immunosorbent assay (ELISA).Results The length and body mass of rats in three groups before and after experiment were not statistically different (P ＞0.05 ).Before the experiment the serum IGF-1 and IGFBP-3 levels were not significantly different(P ＞ 0.05).35 d after the experiment,the serum IGF-1 ((-x)±s,the same below) in the control group,mild hypoxia and severe hypoxia were ( 60.0 ± 18.5 ) ng/ml,(40.6 ± 9.9 ) ng/ml and (13.1 ±8.6)ng/ml,F =25.840,P＜0.01; the serum IGFBP-3 were (1.93 ±0.23).μg/ml,(1.39 ± 0.30) μg/ml and ( 0.90 ± 0.21 ) μg/ml,F =33.929,P ＜ 0.01.The differences were statistically significant.The IGF-1 and IGFBP-3 levels decreased as the hypoxia increased ( P ＜ 0.05 ).Conclusion In simulated sleep apnea hypopnea syndrome,the intermittent hypoxia in young rats does not show physical growth retardation,but the serum IGF-1,IGFBP-3 levels decreased with the increase of hypoxia and decline of oxygen saturation.
