Isolation and in vivo tumorigenicity assay of CD133 + side population cells from laryngeal cancer cell line
10.3760/cma.j.issn.1673-0860.2012.03.010
- VernacularTitle:喉癌CD133+侧群细胞的分离及体内致瘤性分析
- Author:
Chun-Ping WU
1
;
Liang ZHOU
;
Ming XIE
;
Lei TAO
;
Ming ZHANG
;
Jie TIAN
Author Information
1. 复旦大学附属眼耳鼻喉科医院
- Keywords:
Laryngeal neoplasms;
Neoplastic stem cells;
Antigen,CD;
Side-population cells;
Carcinogenicity tests
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(3):223-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a valuable strategy for further purifying cancer stem cells (CSCs) from laryngeal cancer cell line.Methods CD133 + side population (SP) and CD133- SP cells were detected and isolated from laryngeal cancer Hep-2 cell line with SP discrimination and CD133 surface marker,assisted by fluorescence activated cell sorting technology.Freshly sorted CD133 + SP and CD133 - SP cells were xenografted into the subcutaneous space of the right axillary fossa of NOD/SCID mice and tumorigenic capacity of the cells from two subgroups were examinated.Cell cycle distributions of the two cell populations were detected.Results CD133 + SP and CD133- SP cells accounted for (0.30 ± 0.12)％ and ( 17.52 ± 1.59) ％ in Hep-2 cell line,respectively.CD133 + SP cells formed tumor nodules in 15 of 16 mice and CD133 - SP cells in 7 of 16 mice ( Fisher's exact test,P ＜0.05).The mean weight of CD133 + SP tumor nodules was (0.36 ±0.15)g and that of CD133 - SP tumor nodules was (0.08 ±0.04) g.The difference was significant ( t =4.64,P ＜ 0.01 ). Cell cycle analysis revealed similar cycle distributions between the two subgroups.Conclusions CD133 + SP cells harbored much more cancer stem-like tumorigenic potential in NOD/SCID mice than CD133- SP cells. The combination of SP discrimination and surface marker selection helped to purify CSCs further from laryngeal cancer cell line.
