Effect of HDAC6 down-regulation on the growth of xenografted human laryngeal carcinoma cell line Hep-2 in nude mice and underlying mechanism
10.3760/cma.j.issn.1673-0860.2012.06.010
- VernacularTitle:下调组蛋白去乙酰化酶6对Hep-2细胞裸鼠移植瘤的抑制作用及其机制
- Author:
Lin-Lin YUAN
1
;
Wei-Hua LOU
;
Jian-Zhong SANG
Author Information
1. 郑州大学附属第一医院
- Keywords:
Laryngeal neoplasms;
Carcinoma,squamous cell;
Cell apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(6):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of histone deacetylation 6(HDAC6) siRNA on the growth of xenografted human laryngeal squamous cell carcinoma cell line Hep-2 in nude mice and underlying mechanism.Methods Laryngeal squamous cell carcinoma cell line Hep-2 cells were subcutaneously injected to the back of nude mice and transplanted tumor model was established after one week.Nude mice was divided into three groups including blank control group,empty vector group and HDAC6 siRNA group,and the tumor growth was observed. Ki-67 proliferation index was detected by immunohistochemistry.Western blot,in situ hybridization and immunohistochemistry were used to detect the mRNA and protein expressions of HDAC6 in xenograft.The expressions of Bcl-2 and Bax proteins were examined by Western blotting.Cell apoptosis was detected by TUNEL.Results The mean volume of xenograft transfected with HDAC6 siRNA was less than that of xenograft transfected with empty vector or that of xenograft with blank control treatment (P ＜ 0.05 ).HDAC6 siRNA effectively down-regulated the expressions of HDAC6 mRNA and the expressions of HDAC6 and Bcl-2 proteins,but up-regulated the expression of Bcl-2 protein in xenografts,with significant differences ( all P ＜ 0.05 ).The proliferation index of Ki-67 in HDAC6 siRNA transfection group was significantly lower than that in blank control group or emptry vector group ( P ＜ 0.05).TUNEL assay demonstrated that HDAC6 evidently evoked cell apoptosis( P ＜ 0.05 ).Conclusion HDAC6 siRNA could effectively inhibited the growth of xenografted human laryngeal carcinoma cell line Hep-2 in nude mice,down-regulate the expressions of HDAC6 and bcl-2,and up-regulate the expression of bax.
