Expressions and clinical significance of high mobility group box-1 mRNA and protein in laryngeal squamous cell carcinoma tissues and serum
10.3760/cma.j.issn.1673-0860.2012.06.011
- VernacularTitle:高迁移率族蛋白1在喉鳞状细胞癌组织及血清中的表达及意义
- Author:
Ying GUO
1
;
Yong LIU
;
Ping-Qing TAN
;
Guo LI
;
Zhong-Wu SU
;
Yong-Quan TIAN
;
Yuan-Zheng QIU
Author Information
1. 中南大学湘雅医院
- Keywords:
Carcinoma,squamous cell;
Laryngeal neoplasms;
High mobility group proteins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(6):487-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA and protein expressions of high mobility group box-1 ( HMGB1 ) in the tumor tissues and sera of patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance. Methods Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were used to detected the expressions of HMGB1 mRNA and protein in the tumors and adjacent normal epithelial tissues in 30 patients with LSCC.Serum HMGB1 protein levels in the patients with LSCC and in 10 healthy volunteers were detected with enzyme-linked immunosorbent adsorption experiment( ELISA ).Results RT-PCR demonstrated that the mean relative mRNA expression levels of HMGB1 (HMGB1/GAPDH) in LSCC tissues and in adjacent normal epithelial tissues were 1.25 ±0.12 and 0.32 ± 0.04,respectively ( t =40.27,P ＜ 0.05 ).Western blot revealed that the mean relative protein expression levels of H MGB 1 ( HMGB1/β-actin) were 1.29 ± 0.10 and 0.34 ± 0.03 (t =49.84,P ＜ 0.05 ),respectively.Both mRNA and protein expression levels of HMGB1 were associated with T stage,clinical stage,lymph node metastasis status and smoking ( all P ＜ 0.05 ),but no significant correlation with age,alcohol consumption and primary tumor grade and location (all P ＞0.05 ).Mean serum HMGB1 protein levels in patients with LSCC and healthy volunteers were (24.80 ± 14.08) ng/ml and (23.58 ± 14.69) ng/ml ( t =0.37,P ＞ 0.05 ).Conclusions Both mRNA and protein expressions of HMGB1 were obviously elevated in LSCC,which were associated closely with T stage,clinical stage and lymph node metastasis.
