Therapeutic effects of photocatalytic nano-TiO2 on human nasopharyngeal carcinoma xenografts in nude mice
10.3760/cma.j.issn.1673-0860.2012.07.012
- VernacularTitle:光催化纳米二氧化钛对人鼻咽癌裸鼠模型的治疗研究
- Author:
Wei-Jing BAO
1
;
Cong LIN
;
Zhi-Chun LI
;
Chang LIN
;
Min-Xia WU
;
Xiu-Rong ZHONG
Author Information
1. 福建医科大学附属第一医院
- Keywords:
Catalysis;
Titanium;
Nasopharyngeal neoplasms;
Nanotechmology;
Disease models,animal;
Caspase 3;
Apoptosis
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(7):581-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effect of photocatalytic nano-TiO2 on nasopharyngeal carcinoma xenograft in nude mice and underlying mechanism. Methods Nude mice bearing human nasopharyngeal carcinoma xenograft were randomly divided into six groups:nano-TiO2 + UV irradiation( with gradient concentration of nano-TiO2 ) ; nano-TiO2 alone and UV irradiation alone and blank control.The nano-TiO2 suspension was injected into xenografts,and 24 h after UV light with the wave length of 330 -400 nm,all the xenografts were removed and sectioned for HE staining.Ultrastructure and apoptosis of tumor cells in the xenografts were observed by transmission electron microscope (TEM).The expression of Caspase-3 was examined immunohistochemical staining and the apoptosis was detected with TUNEL.Results Pathological analysis showed significant inflammatory responses ( grade Ⅱ and Ⅲ ) with local necrosis occurred in tumor tissues after nano-TiO2 photodynamic therapy,but not in the negative control and blank control.TEM showed the nano-TiO2 particles entered into the cytoplasm and the nucleus of tumor cells and many tumor cells had morphological changes for apoptosis.Significant positive expression of Caspase-3 and TUNEL-positive cells were found in the the xenograts with the treatments of nano-TiO2 + UV irradiation compared to control( P ＜0.01 ),which were enhanced with the increases in nano-TiO2 concentration (P ＜ 0.01 ).Conclusion Photocatalytic nano-TiO2 can inhibit the growth of nasopharyngeal carcinoma xenograft in nude mice by inducing Caspase-3 expression and apoptosis in the tumor cells.
