Neuroprotective Effects of Intraischemic(1 hour) Moderate Hypothermia in Gerbil Brain Global Ischemic Model.
- Author:
In Byung KIM
;
Kyeong Cheon JUNG
;
Sung Vin YIM
;
Seok Joon JANG
;
Seung Whan KIM
- Publication Type:Original Article
- MeSH:
Apoptosis;
Body Temperature;
Brain*;
Carotid Arteries;
Cytoplasm;
DNA Nucleotidylexotransferase;
Eosinophilia;
Gerbillinae*;
Hypothermia*;
In Situ Nick-End Labeling;
Microscopy;
Models, Animal;
Neurons;
Neuroprotective Agents*
- From:Journal of the Korean Society of Emergency Medicine
1999;10(3):350-362
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In animal models of cerebral ischemic-reperfusion has been shown to have a beneficial effect. The object of this study is to compare the effect of pathologic findings between normotheimic and moderate hypothermic group. METHODS: We investigated the effect of moderate hypothermia induced 1 hour after transient(10 min) both carotid artery occlusion on the extent of ischemic-reperfusion cell damage in Mongolian Gerbil model. The terminal deoxyribonucleotidyl transferase (TdT) -mediated biotin-16-dUTP nick-end labelling(TUNEL staning) are used to detect apoptosis. RESULTS: 1. We suggest that Core body temperature is down to moderate hypothermia(30-32degrees C) beyond 10 minite by selective bain cooling method in Mongolian Gerbil model. 2. By light microscopy, ischemic-reperfusion damage were detected in the hippocampal CA1 pyramidal layer on the 3 day after transient ischemic insult, which showed chrosomal condensation and cytoplasmic eosinophilia. Ischemic-reperfusion cells were increased in the CA1 region on the 5 day. Apoptotic cells of the CA1 neurons seen by TUNEL staining than ischemic neurons seen by Hematoxylin-eosin staining were investigate 3 and 5 days after ischemic-reperfusion insult. CONCLUSION: We suggest that is not neuroprotective effects of Intraischemic(1 hour) moderate hypothermia in Gerbil brain global ischemic-reperfusion model.
