Rho-associated coiled kinase inhibitor Y-27632 promotes neuronal-like differentiation of adult human adipose tissue-derived stem cells.
- Author:
Zhong-wen XUE
1
;
Xiao-ming SHANG
;
Hong XU
;
Song-hui LÜ
;
Tian-wei DONG
;
Chao-hui LIANG
;
Yuan YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; cytology; Adult; Amides; pharmacology; Cell Differentiation; drug effects; Cells, Cultured; Female; Humans; Neurons; cytology; Pyridines; pharmacology; Stem Cells; cytology; drug effects
- From: Chinese Medical Journal 2012;125(18):3332-3335
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDY-27632 is a specific inhibitor of Rho-associated coiled kinase (ROCK) and has been shown to promote the survival and induce the differentiation of a variety of cells types. However, the effects of Y-27632 on adult human adipose tissue-derived stem cells (ADSCs) are unclear. This study aimed to investigate the effects of Y-27632 on the neuronal-like differentiation of ADSCs.
METHODSADSCs were isolated from women undergoing plastic surgery and cultured. ADSCs were treated with different doses of Y-27632 and observed morphological changes under microscope. The expression of nestin, neuron specific enolase (NSE) and microtubule-associated protein-2 (MAP-2) in ADSCs treated with Y-27632 was detected by immunocytochemistry and Western blotting analysis.
RESULTSY-27632 had the potency to induce neuronal-like differentiation in ADSCs in a dose-dependent manner. Moreover, the differentiation induced by Y-27632 was recovered upon drug withdraw. ADSCs treated with Y-27632 expressed neuronal markers such as NSE, MAP-2 and nestin while untreated ADSCs did not express these markers.
CONCLUSIONSelective ROCK inhibitor Y-27632 could potentiate the neuronal-like differentiation of ADSCs, suggesting that Y-27632 could be utilized to induce the differentiation of ADSCs to neurons and facilitate the clinical application of ADSCs in tissue engineering.