Prenatal exposure to lipolysaccharide result in expression changes of myocardial renin angiotensin system in offspring rats
10.3760/cma.j.issn.0253-3758.2014.05.015
- VernacularTitle:孕期炎症刺激对子代大鼠心脏局部肾素血管紧张素系统的影响
- Author:
Yujie TANG
1
;
Jianzhi ZHOU
;
Xiaohui LI
Author Information
1. 第三军医大学药学院药物研究所
- Keywords:
Inflammation;
Heart;
Renin-angiotensin system
- From:
Chinese Journal of Cardiology
2014;42(5):418-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore expression changes of myocardial renin angiotensin system induced by prenatal exposure to lipopolysaccharide in offspring rats.Methods Twelve pregnant SD rats were randomly divided into three groups:LPS model group:intraperitoneal injection of LPS (0.79 mg/kg)at 8,10,12 days of pregnancy ; control group:intraperitoneal injection of sterile saline (0.5 ml) at 8,10,12 days of pregnancy; LPS + PDTC group:intraperitoneal injection of LPS (0.79 mg/kg) at 8,10,12 days of pregnancy plus daily intraperitoneal injection of NF-κB inhibitor-pyrrolidine dithiocarbamate (PDTC,100 mg/kg) on day 8 to 14 pregnancy day.Protein expression of Angiotensin Ⅱ (Ang Ⅱ) in heart was detected by immunohistochemistry; myocardial ACE,ACE2 mRNA expression was detected by real-time PCR; protein expression of ACE and ACE2 in heart was detected by Western blot in offspring rats of various groups.Results Compared with control group (0.07 ± 0.02,0.11 ± 0.01),Ang Ⅱ protein levels (0.14 ± 0.04) were significantly increased at 6 weeks (P ＜ 0.01) and 16 weeks (0.17 ± 0.04,P ＜ 0.05) in offspring rats of LPS model group,which could be significantly attenuated by PDTC intervention (0.10 ± 0.01,0.13 ± 0.03,respectively,all P ＜ 0.05).Similarly,myocardial ACE mRNA expression in 16 weeks offspring rats of LPS model group was significantly upregulated compared with control group (1.10 ± 0.26 vs.0.72 ± 0.22,P ＜ 0.05),which was significantly attenuated by PDTC intervention (0.67 ± 0.01,P ＜ 0.01 vs.LPS group).Myocardial protein expression ACE2 in 16 weeks offspring rats of LPS model group was significantly downregulated compared to control group,which was slightly upregulated by PDTC intervention (P ＞ 0.05).Conclusion Pregnancy exposure to lipopolysaccharide increases myocardial ACE and Ang Ⅱexpression while reduces myocardial ACE2 expression in offspring rats,which might be one of the pathomechanisms of offspring hypertension.
