Construction of a eukaryotic expression plasmid of Humanin.
- Author:
Ben-yan LUO
;
Xiang-ming CHEN
;
Min TANG
;
Feng CHEN
;
Zhi CHEN
- Publication Type:Letter
- MeSH: Base Sequence; Cloning, Molecular; methods; Escherichia coli; genetics; metabolism; Humans; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Molecular Weight; Plasmids; genetics; Protein Engineering; methods; Proteins; chemistry; genetics; metabolism; Recombinant Proteins; biosynthesis; chemistry
- From: Journal of Zhejiang University. Science. B 2005;6(1):11-13
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.
METHODSThe recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.
RESULTSRecombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.
CONCLUSIONSA eukaryotic expression plasmid of Humanin was successfully constructed.