Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS.
- Author:
Chun-xiu PAN
1
;
Xiu-zhu XU
;
Hong-mei HE
;
Xiao-jun CAI
;
Xue-jun ZHANG
Author Information
1. Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
- Publication Type:Journal Article
- MeSH:
Acetamides;
analysis;
chemistry;
Butylene Glycols;
analysis;
chemistry;
Chemical Fractionation;
methods;
Chromatography, High Pressure Liquid;
methods;
Isomerism;
Mass Spectrometry;
methods;
Piperidines;
analysis;
chemistry;
Pyridines;
analysis;
chemistry
- From:
Journal of Zhejiang University. Science. B
2005;6(1):74-78
- CountryChina
- Language:English
-
Abstract:
The cis and trans isomers separation of 2-butene-1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,S)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.
