Representative appressorium stage cDNA library of Magnaporthe grisea.
- Author:
Jian-ping LU
1
;
Tong-bao LIU
;
Xiao-yun YU
;
Fu-cheng LIN
Author Information
1. School of Life Sciences, Zhejiang University, Hangzhou 310029, China.
- Publication Type:Journal Article
- MeSH:
Cloning, Molecular;
methods;
DNA, Fungal;
genetics;
Gene Expression Profiling;
methods;
Gene Expression Regulation, Fungal;
Gene Library;
Magnaporthe;
genetics;
Sequence Analysis, DNA;
methods
- From:
Journal of Zhejiang University. Science. B
2005;6(2):132-136
- CountryChina
- Language:English
-
Abstract:
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.
