The usefulness of p16INK4a in cytological screening of cervical carcinoma.
- Author:
Min LI
1
;
Jian CAO
;
Nai-peng WANG
;
Long-yu LI
;
Ling LI
;
You-lin QIAO
;
Qin-jing PAN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; diagnosis; metabolism; virology; Cervical Intraepithelial Neoplasia; diagnosis; metabolism; virology; Cyclin-Dependent Kinase Inhibitor p16; analysis; Cytodiagnosis; DNA, Viral; analysis; Female; Humans; Immunohistochemistry; Papillomaviridae; genetics; isolation & purification; Papillomavirus Infections; diagnosis; metabolism; virology; Predictive Value of Tests; Uterine Cervical Dysplasia; diagnosis; metabolism; virology; Uterine Cervical Neoplasms; diagnosis; metabolism; virology
- From: Chinese Journal of Oncology 2006;28(9):674-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the value of p16INK4a immuncytochemical examination in cytological screening of cervical carcinoma and precancerous lesions.
METHODSp16JNK4a immuncytochemical detection was performed on 220 specimens remaining from liquid-based cytology, followed up by biopsy histology , and compared with the results of high-risk human papillomavirus ( HR - HPV ) DNA tests . Results In patients with cytological diagnosis of squamous cell carcinoma( SCC) , high-grade squamous intraepithelial lesion (HSIL) , low-grade squamous intraepithelial lesion (LSIL) , atypical squamous cells-cannot exclude HSIL (ASC-H) , and atypical squamous cells of undetermined significance (ASC-US) , the positive rates of p16INK4a were 100.0% (7/7), 92. 2% (107/116) , 24. 3% (17/70) , 100. 0% (14/14) and 36.4% (4/ 11) , respectively. In 111 of the 150 p6INK4a positive cases, we found 97 (87.4% ) cases which had biopsy diagnosises of > or =CIN2, but none in 18 of 70 p16INK4a negative cases was. The difference in the positive rates for p16INK4a between cervical intraepithelial neoplasia (CIN) 1 and > or =CIN2 lesions had statistical significance (P < 0. 01) , whereas for HR-HPV DNA test it was not.
CONCLUSIONp16LNK4a is over-expressed in a HSIL, and it may be useful in cytological screening of high risk patients.
