Association of genetic polymorphisms in the peroxisome proliferator-activated receptor-gamma and methylenetetrahydrofolate reductase with preeclampsia in Korean women.
- Author:
So Hyun LEE
1
;
Bo Hyun PARK
;
Mi Hye PARK
;
Hyesook PARK
;
Sun Hee CHUN
;
Jung Ja AHN
;
Hyun Mee RYU
;
Kwang Soo LEE
;
Hyun Young PARK
;
Young Ju KIM
Author Information
1. Department of Obstetrics and Gynecology, Ewha Medical Research Institute, College of Medicine, Ewha Womans' University, Seoul, Korea. kkyj@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Preeclampsia;
Single nucleotide polymorphism;
PPAR gamma;
MTHFR;
Haplotypes
- MeSH:
DNA;
Female;
Gene Frequency;
Genotype;
Haplotypes;
Humans;
Linkage Disequilibrium;
Logistic Models;
Methylenetetrahydrofolate Reductase (NADPH2)*;
Peroxisomes*;
Polymorphism, Genetic*;
Polymorphism, Single Nucleotide;
PPAR gamma;
Pre-Eclampsia*;
Pregnant Women;
Wills
- From:Korean Journal of Obstetrics and Gynecology
2007;50(4):601-611
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate whether polymorphisms of genes encoding peroxisome proliferator-activated receptor-gamma (PPAR gamma) and methylenetetrahydrofolate reductase (MTHFR) are associated with preeclmapsia in Korean women and also to demonstrate whether there is any haplotypic association between preeclampsia and those genes. METHODS: DNA was extracted from whole blood of 226 preeclampsia patients and 235 healthy pregnant women. The genotypes of SNPs in PPAR gamma (-796A>G, P12A (C>G), H447H (161C>T)) and MTHFR (A222V (677C>T), E429A (1298A>C), R594Q (1793G>A)) were analyzed by a single base primer extension assay using a SNaPShot assay kit. Results were analyzed with the Student's t-test, Chi-square test, and Logistic regression analysis. Haplotype analyses were performed using Haploview 3.2 version. RESULTS: There were no significant differences in genotype or allele frequencies of PPAR gamma and MTHFR gene polymorphisms between preeclampsia patients and controls (p>0.05). No increase in the risk of preeclampsia for those genes was observed under any model of inheritance. Among SNPs of the PPAR gamma, MTHFR genes, only SNPs in MTHFR gene (677C>T, 1298A>C, 1793G>A) were in a strong linkage disequilibrium with each other (Lod score>2.0), but there were no significant differences in genotype distribution of haplotypes of MTHFR gene (TAG, CAG, CCA, CCG) between preeclampsia patients and controls (p>0.05). No statistically significant associations were observed between any haplotypes of MTHFR gene and preeclampsia risk. CONCLUSION: This study suggest that SNPs in PPAR gamma and MTHFR gene were not associated with preeclampsia in Korean women, and its haplotypes were also not associated with preeclampsia.
