Serum proteomic patterns may assist the diagnosis of solitary pulmonary nodules.
- Author:
Xiao-Yang LUO
1
;
Hai-Quan CHEN
;
Yi-Hua SUN
;
Jian-Hua ZHOU
;
Wen-Fing WANG
;
Ping LIAO
;
Cui-Qin XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; blood; diagnosis; Adult; Aged; Biomarkers, Tumor; blood; Blood Proteins; analysis; Diagnosis, Differential; Female; Humans; Lung Neoplasms; blood; diagnosis; Male; Middle Aged; Protein Array Analysis; methods; Proteomics; methods; Sensitivity and Specificity; Solitary Pulmonary Nodule; blood; diagnosis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods
- From: Chinese Journal of Oncology 2006;28(12):907-910
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen relatively specific biomarkers in serum from lung adenocarcinoma patients by surface-enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOFMS), and to investigate the clinical value of SELDI-TOF-MS in differentiation of benign from malignant solitary pulmonary nodules (SPN).
METHODSSerum samples from 71 lung adenocarcinoma patients and 71 healthy volunteers with matched gender, age and history of smoking were analyzed using WCX2 ProteinChip to screen potential biomarkers. 28 patients received surgical treatment among total 53 patients with SPN. The clinical value of SELDI-TOF-MS in differentiation of benign from malignant solitary pulmonary nodules was evaluated by pathological diagnosis.
RESULTSFive highly expressed potential biomarkers were identified with the relative molecular weights of 4047.79 Da, 4203.99 Da, 4959. 81 Da, 5329. 30 Da and 7760.12 Da. The postoperative pathologic diagnosis was lung adenocarcinoma in 24 patients with SPN, validating the clinical value of the 5 potential biomarkers.
CONCLUSIONSELDI-TOF-MS technology is a quick, easy, convenient, and high-throughput analyzing method capable of screening several relatively specific potential biomarkers from the serum of lung adenocarcinoma patients and may have attractive clinic value in differentiation of solitary pulmonary nodules.
