Anti-tumor effects induced by fusion of interleukin-18 gene transfected NCI-H460 lung cancer cell line with dendritic cells.
- Author:
Zai-yun ZHANG
1
;
Jin-min WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cancer Vaccines; administration & dosage; genetics; immunology; Cell Fusion; Cell Line, Tumor; Cell Proliferation; Cells, Cultured; Dendritic Cells; cytology; immunology; Female; Humans; Immunotherapy, Adoptive; methods; Interleukin-18; genetics; metabolism; Lung Neoplasms; immunology; pathology; therapy; Lymphocyte Activation; Male; Mice; Mice, Inbred BALB C; Mice, Nude; T-Lymphocytes; cytology; immunology; T-Lymphocytes, Cytotoxic; immunology; Transfection; Xenograft Model Antitumor Assays; methods
- From: Chinese Journal of Oncology 2007;29(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the anti-tumor effects induced by fusion of interleukin (IL)-18 gene transfected lung cancer cell line NCI-H460 cells with dendritic cells (DC).
METHODS(1) DC were induced from human monocytes and fused with IL-18 transfected NCI-H460 cells. Fusion was selected using MACS microbeads. (2) Four groups (group GT, group PT, group NT and group BC) were set up. T cells activated by IL-18 gene transfected fusion or pcDNA3. 1 + vector transfected fusion and non-transfected fusion were taken as effetor cells. No effector cells was in group BC. Lactic dehydrogenase ( LDH) method was used to evaluate the antitumor effect in vitro. (3) Tumor-bearing nude mice were inoculated with effector cells mentioned above. The tumor size and weight in the 4 groups were compared.
RESULTSThe killing rate in vitro of 3 groups were 53. 14% ,30. 10% and 31.49% , respectively. The tumor size and weight in the 3 groups were lower than group BC, among which group GT was the lowest.
CONCLUSIONFusion of IL-18 gene transfected NCI-H460 lung cancer cells with dendritic cells can effectively induce anti-tumor immunity in the host.
