Relationship between the expression of human leukocyte antigen-b in peripheral blood leukocytes and the differentiation and metastasis of gastric carcinoma.
- Author:
Yi ZHANG
1
;
Xiong ZOU
;
Xiao-jing YANG
;
Xiao-li LI
;
Sheng-mei ZHAO
;
Yan-jing GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cell Differentiation; Female; Flow Cytometry; Gene Expression Regulation, Neoplastic; HLA-B Antigens; analysis; genetics; Humans; Leukocytes; immunology; metabolism; Lymphatic Metastasis; Male; Middle Aged; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms; genetics; immunology; pathology
- From: Chinese Journal of Oncology 2007;29(1):49-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the expression of host human leukocyte antigen-B mRNA (HLA-B mRNA) and HLA-B antigen in peripheral blood leukocytes (PBLs) and the differentiation and metastasis of gastric carcinoma (GC).
METHODSTo design and screen specific primers of HLA-B gene independently, detect the expression of HLA-B mRNA from 30 GC patients by reverse transcription-PCR and compare with the HLA-B antigen expression measured by flow cytometry.
RESULTSThe expression rate of PBL HLA-B mRNA from GC patients (23. 3% ) was very significantly lower than that of normals (87. 5% ) (P <0. 01) , especially concerning the poorly differentiated GC patients with lymph node metastasis (16. 0% ). Measured by flow cytometry, the expression percentage of HLA-B antigen of well-differentiated GC patients without lymph node metastasis was 88. 2% , an obviously decreasing tendency was showed in comparison with that in the normal group (98. 8% ) , although the difference was not significant (P = 0. 056) , and the expression percentage in poorly differentiated GC patients with lymph node metastasis(73. 3% )was declined significantly (P <0. 05).
CONCLUSIONThe expression of PBL HLA-B mRNA and HLA-B antigen in GC patients is decreased or lost, and correlated with differentiation and metastasis of the cancer. The expression of PBL HLA-B mRNA may more directly reflect its relationship with the tumor differentiation and metastasis than that of HLA-B antigen.