Influence of Migu capsule and strengthening spleen prescription on the expression of small intestine VDR mRNA in calf muscle on ovariectomized rats.
- Author:
Li-Tong WANG
1
;
Yong-Fang ZHAO
;
Xiang WANG
;
Yu XU
;
Yuan-Chuan CHEN
;
Hong-Sheng ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Density; Capsules; Drugs, Chinese Herbal; pharmacology; therapeutic use; Female; Gene Expression Regulation; drug effects; Intestine, Small; drug effects; metabolism; Muscle, Skeletal; drug effects; metabolism; Osteoporosis; metabolism; prevention & control; Ovariectomy; Prescriptions; RNA, Messenger; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors, Calcitriol; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Spleen; drug effects; metabolism
- From: China Journal of Orthopaedics and Traumatology 2009;22(5):367-370
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Migu capsule and Strengthening Spleen prescriptions on the expression of vitamin D receptor on small intestine and calf muscle of the rat, while observing whether the Chinese medicine complex prescriptions of different effect such as invigorating the kidney and strengthening the spleen had selectivity to expression of the VDR mRNA.
METHODSCopy the model of osteoporosis by ovariectomy operation, the rats were randomly divided into four groups, pseudo-resection group, model group, Migu capsule group and strengthening Spleen prescriptions group. Drug delivery 12 weeks later from operation, stomach lavage last for 12 weeks. Then, to observe the effect of medicine on the rats' bone mineral density, uterus weight, calf muscle/weight, and the VDR mRNA in small intestine and calf muscle through RT-PCR.
RESULTSMigu capsule enhanced bone mineral density (P<0.05), raised calf muscle/weight (P<0.05), up-regulated expression of calf muscle VDR mRNA in the small intestine (P<0.01). Strengthening Spleen prescriptions remained bone mineral density (P>0.05), up-regulated expression of calf muscle VDR mRNA (P<0.01), there was no obvious difference in uterus weight in Migu capsule group and Strengthening Spleen prescription group.
CONCLUSIONMigu capsule and Strengthening Spleen prescriptions can cure osteoporosis by improving the expression of calf muscle VDR mRNA in the small intestine.
