Association between promoter methylation of cyclooxygenase-2 and expression, and precancerous gastric lesions in a high-risk population.
- Author:
Xiao-Rui NIE
1
;
Yang ZHANG
;
Kai-Feng PAN
;
Lian ZHANG
;
Tong ZHOU
;
Ji-You LI
;
Wei-Cheng YOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; China; epidemiology; Cyclooxygenase 2; genetics; metabolism; DNA Methylation; Female; Gastric Mucosa; metabolism; pathology; Helicobacter Infections; epidemiology; metabolism; pathology; Humans; Male; Middle Aged; Promoter Regions, Genetic; Stomach Diseases; epidemiology; metabolism; pathology
- From: Chinese Journal of Preventive Medicine 2009;43(7):571-575
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the relationship between cyclooxygenase-2 (COX-2) methylation and expression, and precancerous gastric lesions.
METHODSMethylation status of COX-2 was evaluated by quantitative denaturing high performance liquid chromatography (DHPLC) in 1201 subjects with different gastric lesions. COX-2 expression was assessed by immunohistochemistry and Helicobacter pylori (H pylori) infection status was determined by 13C-urea breath test (13 C-UBT).
RESULTSThe percent of COX-2 methylation was increased steadily with the severity of gastric lesions, showing 10.6% of which with superficial gastritis/chronic atrophic gastritis (SG/CAG), 11.8% with intestinal metaplasia (IM) and 13.8% with indefinite dysplasia/dysplasia (Ind DYS/DYS) (chi2 = 8.312, P = 0.016). Stratified analysis indicated that the percents of COX-2 methylation in subjects with H pylori negative still increased with the severity of gastric lesions,of 8.8% in SG/CAG, 10.6% in IM and 14.1% in Ind DYS/DYS (chi2 = 6.629, P= 0.036). Moreover,the methylated proportion of COX-2 was negatively associated with the expression in gastric lesions, from 13.3% with mild expression to 7.6% with strong expression (chi2 = 10.400, P = 0.015).
CONCLUSIONOur findings indicated that COX-2 methylation was significantly associated with precancerous gastric lesions and H pylori infection, suggesting that promoter methylation of COX-2 might play an important role in the progression of gastric lesions.