Comparative analysis of measles genome between vaccine strain and wild-type strain in Zhejiang province of China.
- Author:
Chang-ping XU
1
;
Ju-ying YAN
;
Yan FENG
;
Hai-yan MAO
;
Yi-yu LU
Author Information
- Publication Type:Journal Article
- MeSH: Antigenic Variation; China; epidemiology; Comparative Genomic Hybridization; Genome, Viral; Humans; Measles; epidemiology; prevention & control; virology; Measles Vaccine; genetics; Measles virus; classification; genetics; immunology; Sequence Analysis, Protein; Sequence Analysis, RNA
- From: Chinese Journal of Preventive Medicine 2009;43(8):723-726
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the distinction between wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level.
METHODSAfter sequencing of measles wild-stain MVi/Zhejiang. CHN/7.05/4, the distinction between the wild-type strain and the vaccine strain was analysed by MEGA 3.1 software at genome level, and the antigen variation was studied by means of combining the epidemiological data.
RESULTSThere were 822 nucleotide differences (5.17%) and 161 amino acid differences between these two strains, including three glycosylation sites variation found. Meanwhile, the antigen ratio between wild-type strain and vaccine strain was found to be 5.66.
CONCLUSIONThere should be certain differences between the contemporary wild-type strain MVi/Zhejiang, CHN/7.05/4 and vaccine strain Shanghai-191 at genome level, and the protective effects of measles vaccine should be studied further.
