The etiology and clinical manifestations of 70 patients with hand-foot-mouth disease.
- Author:
Xin ZHANG
1
;
Hui-ping YAN
;
Chun HUANG
;
Yu-fen TAN
;
Dong-mei MA
;
Hai-ping ZHANG
;
Yan LIU
;
Shu-zhao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Child; Child, Preschool; Enterovirus; genetics; Female; Hand, Foot and Mouth Disease; etiology; virology; Humans; Infant; Male; RNA, Viral; analysis; Reverse Transcriptase Polymerase Chain Reaction; Serotyping; Young Adult
- From: Chinese Journal of Preventive Medicine 2009;43(10):872-874
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the etiology and clinical manifestations of 70 patients with hand-foot-mouth disease (HFMD).
METHODSThe viral RNA from the pharynx swab samples were extracted and collected from 70 HFMD patients (of which, 60 cases were under 5 years old) to detect the 5'-UTR gene of EV, the Vp3-Vp1 genes of EV71 and Cox-A16 by utilizing the technique of RT-PCR.
RESULTSOf 70 HFMD patients, 30 cases (42.8%, 30/70) were positive in enterovirus nucleic acid, including EV71 and Cox-A16, pharynx swab sample testing. While, of the enteroviral pathogen RNA-positive 30 cases, EV71 accounted for 66.7% (20/30). The proportion of etiological positive of 39 samples collected within 4 days after onset was 66.7% (26/39), but the probability of 31 samples collected after more than 5 days after onset was 12.9% (4/31), the difference was significantly (chi(2) = 20.4, P < 0.01).
CONCLUSIONHFMD patients were mainly infants and young children between 0-5 years old, but adults could also be attacked. The enterovirus detected was mainly EV71, and the pharynx swab samples should be collected within 4 days after onset to increase the related viral nucleic acid positive detection probability.
