Study on the automatic ribotyping for Enterobacter sakazakii.
- Author:
Xiao-yan PEI
1
;
Yun-chang GUO
;
Xiu-mei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cronobacter sakazakii; classification; genetics; isolation & purification; DNA, Bacterial; DNA, Ribosomal; Food Microbiology; Ribotyping; methods
- From: Chinese Journal of Preventive Medicine 2009;43(10):900-902
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the ribotyping fingerprint of Enterobacter sakazakii (E. sakazakii) isolated from food and its typing power.
METHODSTwo standard strains and twenty-eight isolates of E.sakazakii were analyzed by the DuPont Riboprinter(TM) microbial characterization system. The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software.
RESULTSThis system grouped two standard strains and twenty-eight E.sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively, the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters.
CONCLUSIONThe automatic ribotyping method is convenient and fast in E.sakazakii typing.
