Inhibitory effect of weikangfu recipe on growth of mouse S180 tumor and its apoptotic induction.
- Author:
Xiao-Hua NIE
1
;
Bao-Jun SHI
;
Wen-Yi TAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Cycle; drug effects; Dose-Response Relationship, Drug; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Female; Male; Mice; Neoplasm Transplantation; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Random Allocation; Sarcoma 180; pathology; Tumor Suppressor Protein p53; metabolism; bcl-2-Associated X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2006;31(17):1457-1460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the growth inhibition of Weikangfu recipe on S180 tumor and its apoptotic induction.
METHODS180-bearing mice were orally administrated with different dosages of Weikangfu recipe, and the growth inhibition was evaluated; apoptotic cells induced were detected by flow cytometry and DNA agarose gel electrophoresis.
RESULTWeikangfu recipe showed significant inhibition on the growth of S180 tumor in a dose-dependent manner, compared with the control group. From apoptotic analyses, Weikangfu recipe induced a dose-dependent apoptosis of S180 tumor cells and arrested the cell cycle distribution at G0-G1 phase. At the same time, the up-regulation of p53 and bax and down-regulation of bcl-2 were observed in S180 tumor cells of the treated groups.
CONCLUSIONOur findings demonstrate that Weikangfu recipe can significantly inhibit the growth of S180 tumor and induce apoptosis through expression alteration of p53, bax and bcl-2.
