Effect of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension culture.
- Author:
Cong-Bing FANG
1
;
He-Qin LI
;
Xiao-Chun WAN
;
Chang-Jun JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; drug effects; Cells, Cultured; Charcoal; pharmacology; Culture Media; Culture Techniques; Hydrogen-Ion Concentration; Isoflavones; metabolism; Plants, Medicinal; cytology; growth & development; metabolism; Polystyrenes; Polyvinyls; Pueraria; cytology; growth & development; metabolism; Sucrose; pharmacology
- From: China Journal of Chinese Materia Medica 2006;31(19):1580-1583
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo illustrate the effects of several physiochemical factors on cell growth and isoflavone accumulation of Pueraria lobata cell suspension cultures.
METHODHigh performance liquid chromatography and plant tissue culture were applied.
RESULT AND CONCLUSIONCell growth and isoflavone accumulation were significantly stimulated in P. lobata cell suspension cultures by the increase of the sucrose concentration. Maintaining the pH value at the range over 5. 4 to 5. 8 was most suitable for isoflavone accumulation in P. lobata cell suspension cultures. Cell dried weight and isoflavone accumulation decreased sharply with the increase of the treated concentration of active carbon, while XAD-4 significantly stimulated cell growth and isoflavone accumulation.
