Genetic diversity of Eucommia ulmoides by RAPD analysis.

Author: Ai-Qi WANG ¹ ; Lu-Qi HUANG ; Ai-Juan SHAO ; Guang-Hong CUI ; Min CHEN ; Chang-Hui TONG
Author Information

1. Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
MeSH: China; Conservation of Natural Resources; DNA, Plant; genetics; Eucommiaceae; classification; genetics; Genetics, Population; Phylogeny; Plants, Medicinal; genetics; Polymorphism, Genetic; Random Amplified Polymorphic DNA Technique
From: China Journal of Chinese Materia Medica 2006;31(19):1583-1586
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo determine the genetic diversity of Eucommia ulmoides.
METHOD260 samples of 20 populations were analyzed through radom amplified polymorphic DHA (RAPD).
RESULTTotal polymorphic loci percentage was 96.36 and the average was 38.92. 110 bands were produced with 10 random primers and 106 were polymorphic. Nei's gene diversity (H) was 0.246 1, Shannon's Information index(I) was 0.386 8, Gst was 0.424 4, indicating that 42.44% of the genetic variation was distributed among populations and 57.65% within populations.
CONCLUSIONThe genetic variation was relatively high in E. ulmoides, so the genetic diversity conservation principle should mainly focus on protection of the populations.