Studies on extraction, isolation and composition of Lycium barbarum polysaccharides.
- Author:
Meili TIAN
1
;
Min WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; analysis; Hexuronic Acids; analysis; Lycium; chemistry; Monosaccharides; analysis; Plants, Medicinal; chemistry; Polysaccharides; chemistry; isolation & purification; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(19):1603-1607
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP).
METHODLBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc.
RESULTPure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids.
CONCLUSIONLBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
