Effects of tetramethylpyrazine on expression of vascular endothelial growth factor and hypoxia-induced factor-1alpha by rat peritoneal macrophages.
- Author:
Xiao-Yu XU
1
;
Lan YE
;
Gang CHEN
;
Jun-Dong FENG
;
Wei-Hai CHEN
;
Yi-Yong HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Ligusticum; chemistry; Macrophages, Peritoneal; cytology; metabolism; Male; Plants, Medicinal; chemistry; Pyrazines; isolation & purification; pharmacology; Rats; Rats, Wistar; Vascular Endothelial Growth Factor A; biosynthesis
- From: China Journal of Chinese Materia Medica 2006;31(19):1608-1611
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of tetramethylpyrazine on lipopolysaccharides (LPS)-induced expression of vascular endothelial growth factor (VEGF) and hypoxia-induced factor-1alpha (HIF-1alpha) in macrophages.
METHODRat peritoneal macrophages were treated with LPS, and at the same time given different doses of tetramethylpyrazine. The secreation of VEGF was determined by ELISA test, and MTT assay was used to examine cells proliferation. Western blot assay was used to examine the expression of HIF-1alpha.
RESULT100 microg x mL(-1) and 10 microg x mL(-1) tetramethylpyrazine decreased the secretion of VEGF and also inhibited the expression of HIF-1alpha by LPS-induced macrophages. But these doses of tetramethylpyrazine had no effect on the cells proliferation.
CONCLUSIONTetramethylpyrazine could inhibit the secretion of VEGF by LPS-induced macrophages, and the mechanism must be associated with inhibiting the expression of HIF-1alpha. The inhibition effect was not due to inhibition of the proliferation of macrophages.
