Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths.
- Author:
Xin-Zhong LI
1
;
Peng LEI
;
Shao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Berberine; analogs & derivatives; analysis; Berberine Alkaloids; analysis; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavonoids; analysis; Iridoids; analysis; Plants, Medicinal; chemistry; Pyrans; analysis; Quality Control; Ultraviolet Rays
- From: China Journal of Chinese Materia Medica 2006;31(20):1686-1688
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.
METHODA HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).
RESULTThis method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.
CONCLUSIONThe quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.
