Study on bile excretion of scutellarein.
- Author:
Qing-Ming CHE
1
;
Ying CHEN
;
Li-Yi PAN
;
Hong HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apigenin; analysis; blood; isolation & purification; Bile; chemistry; Chromatography, High Pressure Liquid; Erigeron; chemistry; Glucuronates; analysis; Male; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2006;31(20):1710-1712
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA HPLC-ECD method was established to determine scutellarin in rat bile.
METHODThe analytical column was Prontosil C18 (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol, 50 mmol x L(-1) phosphate buffer (adjusted by phosphoric acid to pH 2. 6), and tetrahydrofuran (40: 60: 10) , the flow rate was 1.0 mL x min(-1); the potential electrode voltage was 100 mv.
RESULTConcentration profile of scutellarin in rat bile was shown in this paper after oral administration of scutellarein.
CONCLUSIONOnly scutellarin was detected in rat bile, while both of scutellarin and scutellarein were detected in rat plasma.
