Determination of astragulin in Thesium chinese by RP-HPLC.
- Author:
Yang LIU
1
;
Yu-Lin DENG
;
Yu-Hong YU
;
Rong-Ji DAI
;
Zhi-Hui XU
;
Wei-Wei MENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Flavonoids; analysis; Glucosides; analysis; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Santalaceae; chemistry
- From: China Journal of Chinese Materia Medica 2006;31(21):1766-1767
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a determination method of astragulin in Thesium chinese.
METHODRP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times.
RESULTThe content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location.
CONCLUSIONThe method was validated to show convenient and reliable.
