Ribosomal DNA ITS sequence analyses of Pinellia ternata from different geographical origin in China.
- Author:
Jun-Yi ZHANG
1
;
Qiao-Sheng GUO
;
Li-Wei WU
;
Yue-Yu HANG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; DNA, Plant; genetics; DNA, Ribosomal Spacer; genetics; Ecosystem; Molecular Sequence Data; Phylogeny; Pinellia; genetics; Plant Tubers; genetics; Plants, Medicinal; genetics; RNA, Ribosomal, 5.8S; genetics; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2006;31(21):1768-1772
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the ITS sequence variation of Pinellia ternata from different population in China, and it correlation to geographical distribution and morpha of the plant.
METHODThe rDNA ITS regions of various P. ternata were amplified and sequenced. And they were analyzed by means of the software of CLUSTRAL and MEGA.
RESULTComplete sequence of ITS and 5.8S rDNA of 16 different P. ternata population were obtained. The sequences of ITS1, 5.8S and ITS2 are 276,162 and 246 bp, respectively. ITS1 was more conservative than ITS2. Phylogenetic tree based on ITS1 and ITS2 sequences data was conducted by Neighbor-joining method.
CONCLUSIONRibosomal DNA ITS sequence analyses can be applied to the resource research of P. ternata.
