Study on influence of buffer system on separate capacity of macroporous resin on ferulic liposome.
- Author:
Bao-Yu CHEN
1
;
Jing QIN
;
Da-Wei CHEN
;
Hai-Yang HU
;
Ya-Shu YIN
;
Kai-Liang YANG
;
Yi-Fan LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adsorption; Buffers; Coumaric Acids; administration & dosage; analysis; Drug Carriers; Liposomes; Quality Control; Resins, Synthetic
- From: China Journal of Chinese Materia Medica 2006;31(21):1780-1783
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of buffer on separate capacity of macroporous resin. To evaluate the quality of ferulic acid liposome and determine its entrapment efficiency.
METHODDifferent type of macroporous resin counterpoised by buffer system of Na2 HPO3-NaH2, PO3 was used to separate the free ferulic acid from the preparation and HPLC was used to determine the concentration of the ferulic acid to calculate the entrapment efficiency.
RESULTThis method had good linearity in the range of 0.56 - 2.8 g x mL(-1) (r = 0.999 6). The precision RSD was less than 1.1%. The adsorption effect of macroporous resin on liposome was reduced while it had no effect on the absorption ability of macroporous resin on the ferulic acid by the usage of buffer. The recovery of HPD450 resin on blank liposome was between 97.2% - 100.8%, while the average recovery is 98.1%.
CONCLUSIONBuffer system can enhance the separate ability of macroporous resin on liposome and free drug.
