Neuro-protective effect of Naomaitong to inflammatory cascade response after focal cerebral ischemia reperfusion in aged rats.
- Author:
Jian-Sheng LI
1
;
Jian-Feng GAO
;
You-Long ZHOU
;
Ke LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; pathology; Brain Ischemia; etiology; metabolism; pathology; Drugs, Chinese Herbal; isolation & purification; pharmacology; Infarction, Middle Cerebral Artery; chemically induced; Intercellular Adhesion Molecule-1; biosynthesis; genetics; Male; Neuroprotective Agents; isolation & purification; pharmacology; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley; Reperfusion Injury; etiology; metabolism; pathology; Tumor Necrosis Factor-alpha; metabolism; Vascular Cell Adhesion Molecule-1; metabolism
- From: China Journal of Chinese Materia Medica 2006;31(21):1804-1807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of Naomaitong on inflammatory cascade response after focal cerebral ischemia reperfusion in aged rats.
METHODWe duplicated focal cerebral ischemia model with MCAO, with ischemia 3 h and I/R 1, 3, 6, 12 d points. The effect of Naomaitong on the nervous dysfunction score, the water content of cerebral constitution and the expression of TNF-alpha, VCAM-1, ICAM-1 and its mRNA were observed, and the group with nimodipine was as control.
RESULTThe nervous dysfunction score (I/R1, 3, 6 d), the water content of cerebral constitution (all the time points), the expression of TNF-alpha, VCAM-1 (I 3 h, I/R 1, 3, 6 d), ICAM-1 (I 3 h,I/R 1, 3, 6 d) and its Mrna (all the time points) in model group were higher than those of the sham-operated group; The nervous dysfunction score, the water content of cerebral constitution (I/R 3, 6, 12 d), the expression of TNF-alpha, VCAM-1 (I 3 h, I/R 1, 3 d), ICAM-1 and its mRNA (I 3 h, I/R 1, 3 d) in model group were decreased compared with that of model group. The nervous dysfunction score (I/R 6, 12 d), the expression of TNF-alpha, ICAM-1 (I/R 1d) and its mRNA (I/R 1, 3 d) in Naomaitong group were higher than that of Nimodipine group.
CONCLUSIONNaomaitong could protect brain cell from damage after focal cerebral ischemia reperfusion injury by inhibiting the expression of TNF-alpha, adhesion molecule.
