Study of Shuangling Fuzheng anti-tumor preparation on proliferation and c-myc gene expression of SGC-7901 cells.
- Author:
Hua-Sheng CHEN
1
;
Ai-Hua XU
;
Lin-Chang JIA
;
Li REN
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antineoplastic; pharmacology; Antineoplastic Agents; pharmacology; Antineoplastic Agents, Phytogenic; administration & dosage; isolation & purification; pharmacology; Cell Line, Tumor; Cell Proliferation; drug effects; Cisplatin; pharmacology; Dose-Response Relationship, Drug; Doxorubicin; pharmacology; Drug Combinations; Drug Synergism; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacology; Gene Expression Regulation, Neoplastic; drug effects; Genes, myc; Humans; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-myc; metabolism; Stomach Neoplasms; metabolism; pathology
- From: China Journal of Chinese Materia Medica 2006;31(21):1822-1824
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups on proliferation and c-myc gene expression of SGC-7901 cells in vitro.
METHODThe inhibitory effect of single SLAP (40 -640 microg x mL(-1)) and combined therapy with adriamycin (0.4, 4.0 microg x mL(-1) or cisplatin (0.1,1.0 microg x mL(-1) on human gastric carcinoma SGC-7901 cells proliferation were observed by MTT colorimetric analysis method. Technique of flow cytometry in vitro was used to measure the rate of positive sign of SLAP (80 - 320 microg x mL(-1)) on c-myc gene protein of SGC-7901 cells.
RESULTSGC-7901 cells proliferation were inhibited by single SLAP in dose of 40 - 640 microg x mL(-1) 24 h. Its inhibitory rate was increased with increase of dose. The inhibitory rate on SGC-7901 cells could be increased by SLAP in dose of 40 - 640 microg x mL(-1) plus adriamycin in dose of 0.4 and 4.0 microg x mL(-1) or plus cisplatin in dose of 0.1 and 1.0 microg x mL(-1). At the same time, SLAP (80 - 320 microg x mL(-1)) also could inhibite the expression of c-myc gene of SGC -7901 cells.
CONCLUSIONSingle SLAP had inhibiting effect on human gastric carcinoma cells proliferation with a dose-effect relationship and synergic effect while combined with adriamycin or cisplatin. To inhibit the expression of c-myc gene of human gastric carcinoma cells might be one of action mechanisms of SLAP, which inhibited tumor cells proliferation.
