Expression of carbonic anhydrase II in cultured rat osteoclasts after stress stimulation.
- Author:
Qiang DONG
1
;
Qian XIA
;
Jianjiang ZHOU
;
Hong MA
;
Yong WANG
;
Xing LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; Carbonic Anhydrase II; Cells, Cultured; Osteoclasts; Polymerase Chain Reaction; RNA, Messenger; Rats; Rats, Sprague-Dawley; Stress, Mechanical
- From: West China Journal of Stomatology 2013;31(4):425-428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test expression of carbonic anhydrase II (Ca II) mRNA in osteoclasts which were applied with fluid shear stress.
METHODSThe bone marrow cells of Sprague-Dawley rats were cultured with the presence of 1,25-(OH)2D3 and dexamethasone. The osteoclast-like cells were identified by tartrate-resistant acid phosphatase(TRAP) staining and scanning electron microscope (SEM) observation, then purified with trypsin/ethylenediamine tetraacetic acid (EDTA). Different values and lasting time of steady fluid shear stress were exerted on the osteoclasts with parallel plate flow system. The Ca II expression of osteoclasts were detected by real time reverse transcription-polymerase chain reaction(RT-PCR) and nested polymerase chain reaction(PCR).
RESULTSThe levels of Ca II mRNA were down-regulated correspondingly with the increase of stress and time (P < 0.05).
CONCLUSIONIt's indicated that steady fluid shear stress within a certain range may down-regulate the expression of Ca II in osteoclasts.
