Inhibitory effects of high mobility group chromosomal protein N2 on human tongue carcinoma transplanted in nude mice.
- Author:
Xiqian LIU
;
Xiaoqian DONG
;
Yonghong ZHANG
;
Ping ZHANG
;
Xiaoyu LI
;
Sixiu CHEN
;
Yun FENG
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma; Cell Line, Tumor; High Mobility Group Proteins; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Tongue Neoplasms
- From: West China Journal of Stomatology 2014;32(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibitory effect of high mobility group chromosomal protein N2 (HMGN2) on human tongue carcinoma tumor in nude mice.
METHODSA transplantation tumor model in nude mice was constructed by injecting Tca8113 cells. After a week, negative control groups, masculine control groups, and HMGN2 groups were established. Cell culture of the three groups were separately injected with washing buffer II, cis-dichlorodiamineplatinum (DDP), and HMGN2 protein. The tumors were moved after four treatments, and then analyzed by hematoxylin-eosin (HE) staining.
RESULTSA transplanted tumor model was established successfully. The volumes of HMGN2 groups and masculine control groups were smaller than those of the negative groups. Mouse weight did not differ among the three groups. Average tumor weight of the negative group was (0.38 +/- 0.19)g, that of the HMGN2 group was (0.21 +/- 0.15)g, and that of the DDP group was (0.23 +/- 0.16)g. These factors indicated no statistically significant difference among the three groups. The tumor inhibitory rate of HMGN2 group was 45.71%, and that of the positive group was 39.44%. Based on evaluation by naked eye, the tumor in the negative group was larger than that in other groups. In addition, cell necrosis was observed during HE staining.
CONCLUSIONHMGN2 could significantly inhibit growth of the transplanted tumor in nude mice.