Study of the biological characteristics of p75 neurotrophin receptor positive tongue squamous cell carcinoma cells.
- Author:
Dongdong TONG
;
Fenghe ZHANG
;
Yao YAO
;
Zhaotao ZHANG
;
Jinbing WANG
;
Qing LI
;
Xinlian ZHANG
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; Humans; Neoplasms; Nerve Tissue Proteins; Receptors, Nerve Growth Factor; Tongue Neoplasms
- From: West China Journal of Stomatology 2014;32(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the biological characteristics of p75 neurotrophin receptor positive (p75(NTR+)) tongue squamous cell carcinoma cells which were separated by flow cytometry cell sorting.
METHODSTo determine the biological characteristics of p75(NTR+) cells which were separated from Tca-8113 and Cal-27 tongue squamous cell carcinoma cells by flow cytometry cell sorting, including study the capacity of cloning, 3-(4,5)-demethylthiazo(z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, wound healing assay. p75(NTR+) cells with non-sorted cells were as control group.
RESULTSIn Tca-8113 and Cal-27 tongue squamous cell carcinoma cell lines, the percentage of p75(NTR+) cells were 3.1% and 1.9%. Compared with p75(NTR+) cells with non-sorted cells, p75(NTR+) cells possess higher capacity of cloning (Tca-8113, P=0.024; Cal-27, P=0.009). The percentage of p75(NTR+) cells of the progeny cells generated from monoclonal p75(NTR+) cells decreased to 14.5% (Tca-8113) and 5.8% (Cal-27) after cultured two weeks. p75(NTR+) cells possessed higher proliferation ability and higher metastasis ability than non-sorted cells.
CONCLUSIONp75(NTR+) cells isolated from tongue squamous cell carcinoma have the characteristics of cancer stem cells.