Effects of Porphyromonas gingivalis infection on intercellular adhesion molecule-1 expression in rat vascular smooth muscle cells.
- Author:
Jiayin DAI
;
Jiang LIN
;
Liangjia BI
;
Lining JIAO
;
Qiang WANG
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Intercellular Adhesion Molecule-1; Muscle, Smooth, Vascular; Myocytes, Smooth Muscle; Porphyromonas gingivalis; RNA, Messenger; Rats
- From: West China Journal of Stomatology 2014;32(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Porphyromonas gingivalis (P. gingivalis) ATCC 33277 infection on expression of intercellular adhesion molecule-1 (ICAM-1) in rat vascular smooth muscle cells(VSMC).
METHODSAn infection model of rat VSMC invaded by P. gingivalis was established in vitro. The mRNA of ICAM-1 was measured through reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSCompared with the control group, an apparent and statistically significant increase in expression of ICAM-1 mRNA was observed after 8, 16, and 24 h in P. gingivals-infected rat VSMC (P<0.05). The expression reached its peak at 16 h. Statistically significant differences were observed in the 8 h group and in the other two experimental groups (P<0.05).
CONCLUSIONInfection of P. gingivals in rat VSMC can cause increased expression of ICAM-1, which may have an important function in the progression of atherosclerosis.