Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles.
- Author:
Hao WU
;
Hong-chen LIU
;
Lingling E
;
Na LIU
;
Bo ZHANG
;
Yuxuan GAO
;
Lin FENG
- Publication Type:Journal Article
- MeSH: Bone and Bones; Cell Differentiation; Humans; Mandible; Osteoblasts; Osteocalcin; Peri-Implantitis; RNA, Messenger
- From: West China Journal of Stomatology 2014;32(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.
METHODSPrimary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.
RESULTS: After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).
CONCLUSIONPeri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.