Changes of hearing threshold and calcium/camodulin in the cochlear nucleus cells of mice with cytomegalovirus intracranial infection
10.3760/cma.j.issn.1673-0860.2013.02.022
- VernacularTitle:颅内感染巨细胞病毒小鼠听反应阈和蜗神经核细胞内游离钙及钙调素的变化
- Author:
Cai-Ji WANG
1
;
Yue-Hua QIAO
;
Qin LI
;
Pei-Hua LI
;
Hong MENG
;
Ling-Jian MENG
;
Xuan-Yi LI
;
Xiao-Lu PEI
Author Information
1. 221006,徐州医学院附属医院耳鼻咽喉头颈外科
- Keywords:
Cytomegalovirus infections;
Hearing loss,sensorineural;
Cochlear nucleus;
Calcium;
Calmodulin
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the threshold of auditory brainstem response (ABR),[Ca2+]i and calmodulin(CaM)in cochlear nucleus of the newborn mice infected by murine cytomegalovirus(MCMV)in the brain.Methods Sixty-nine newborn mice were randomized into the model group and the control group.The model group(54 mice)was established by intracranial injection with MCMV viral suspension 20 μl,and the same volume of 0.9％ sodium chloride was injected in the control group(15 mice).After one month,the ABR was tested in a sound-electric screen environment and the threshold was recorded.Then intracellular free calcium [Ca2 +] i and the mRNA level of CaM in the cochlear nucleus were assayed by flow cytometry and RT-PCR.Results Compared to the control group [(64.0 ±1.3)dBSPL],the threshold of ABR in the model group [(84.5 ±2.7)dBSPL] was increased(F =2.789,P =0.000).Moreover,in the model group the intracellular free calcium [Ca2 +] i and the mRNA level of CaM in the cochlear nucleus were increased(F =1.290,P =0.000;F =4.252,P =0.023),the differences were statistically significant.Conclusions The intracranial injection of MCMV can lead to abnormal changes in the threshold of ABR in mice,and the change of [Ca2 +]i/CaM in cochlear nucleus may be the important pathological basis of sensorineural hearing loss induced by MCMV infection.
