Radiobiological characteristics and MRN complex expression of human nasopharyngeal carcinoma cell lines.
- Author:
Jian-cong HUANG
1
;
Ge-hua ZHANG
;
Jing-jia LI
;
Rui-cheng YAN
;
Xi-fu WU
;
Jian ZHENG
;
Ming-yue LÜ
;
Feng-an XIE
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma; Cell Cycle Proteins; metabolism; Cell Line, Tumor; radiation effects; DNA Repair; DNA Repair Enzymes; metabolism; DNA-Binding Proteins; metabolism; Gene Expression Regulation, Neoplastic; Humans; MRE11 Homologue Protein; Nasopharyngeal Neoplasms; pathology; radiotherapy; Nuclear Proteins; metabolism; Radiation Tolerance
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2013;48(3):195-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the radiobiological characteristic of human nasopharyngeal carcinoma cell lines CNE1 and CNE2 and the changes in expression MRN (Mre11-Rad50-Nbs1) complex in the cell lines exposed to irradiation.
METHODSCNE1 and CNE2 were irradiated by a linear accelerator. Radiobiological characteristics were detected by colony assay and MTT assay. MRN complex expression were examined by Western blot.
RESULTSSurviving fraction at 2 Gy (SF2), quasi-threshold Dose (Dq), and mean lethal dose (Do) of CNE1 were 0.56, 1.449 Gy and 1.480 Gy; SF2, Dq, and Do of CNE2 were 0.44, 0.776 Gy and 1.685 Gy, respectively. Survival fraction of CNE1 at the day 6 after 4 Gy irradiation was 0.59 and that of CNE2 was 0.79 when compared with control, with the up-regulated expressions of Rad50 in CNE1 and Mre11, Rad50 and Nbs1 in CNE2 (P < 0.05).
CONCLUSIONSCNE1 and CNE2 were sensitive to radiation, but there were radioresistance cells in CNE2. The expressions of some components of MRN complex were up-regulated to repair DNA lesions induced by radiation.
