EphA2 mediated vascular endothelial growth factor expression via the p38 MAPK signaling pathway in squamous cell carcinoma of the head and neck.
- Author:
Yong LIU
1
;
Hao-lei TAN
;
Guo LI
;
Chang-yun YU
;
Zhong-wu SU
;
Shu-ling REN
;
Gang-cai ZHU
;
Yuan-zheng QIU
;
Yong-quan TIAN
;
Xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; metabolism; Cell Line, Tumor; Enzyme Inhibitors; pharmacology; Head and Neck Neoplasms; metabolism; Humans; Imidazoles; pharmacology; MAP Kinase Signaling System; Pyridines; pharmacology; Receptor, EphA2; physiology; Vascular Endothelial Growth Factor A; metabolism; p38 Mitogen-Activated Protein Kinases; antagonists & inhibitors; metabolism
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2013;48(3):229-233
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory effect of erythropoietin-producing hepatocellular receptor (EphA2) on the expression of VEGF protein, a pro-angiogenic factor, via p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway in squamous cell carcinoma of the head and neck(SCCHN) in vitro.
METHODSSCCHN Tu686 cells were transfected with EphA2 overexpression vector pEGFP-N1-EphA2. Western blot was used to detect the expression of p38 MAPK and enzyme-linked immunosorbent assay (ELISA) was applied to assay of VEGF. SB203580 as a inhibitor of p38 MAPK signaling pathway was used.
RESULTSThe expression of VEGF protein was significantly up-regulated in Tu686 cells transfected with EphA2 overexpression vector (535.31 ± 45.71) pg/ml, when compared with Tu686 cells transfected with empty vector (400.99 ± 33.50) pg/ml and Tu686 cells with no transfection (385.30 ± 33.50) pg/ml (F = 17.091, P < 0.01). The expression of phosphorylated p38 MAPK was obviously increased in Tu686 cells with EphA2 overexpression. SB203580 inhibited the expressions of VEGF and phosphorylated p38 MAPK proteins in Tu686 cells with EphA2 overexpression.
CONCLUSIONEphA2 can regulate the expression of VEGF protein and stimulate p38 MAPK signaling pathway.