Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecipitation.
- Author:
Fei SHEN
1
;
Zhi-tao LI
;
Yi-ming WU
;
Wei-dong WU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Chromatin Immunoprecipitation; Gene Expression Regulation, Neoplastic; Humans; Polymerase Chain Reaction; Promoter Regions, Genetic; genetics; Protein Binding; Transcription Factor AP-1; genetics; Tumor Suppressor Protein p53; genetics
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(8):453-456
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro.
METHODSAP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment.
RESULTSThe p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.
CONCLUSIONAP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.
