Spectra of Chromosomal Aberrations in 325 Leukemia Patients and Implications for the Development of New Molecular Detection Systems.
10.3346/jkms.2011.26.7.886
- Author:
Hyun Jung CHOI
1
;
Hye Ran KIM
;
Myung Geun SHIN
;
Hoon KOOK
;
Hyeoung Joon KIM
;
Jong Hee SHIN
;
Soon Pal SUH
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Hwasun Hospital, Hwasun, Korea. mgshin@chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Leukemia;
Chromosomal Abnormalities;
Molecular Detection System
- MeSH:
Adaptor Proteins, Signal Transducing/genetics;
Adult;
Aged;
Aged, 80 and over;
*Chromosome Aberrations;
Chromosomes, Human, Pair 14;
Chromosomes, Human, Pair 17;
Chromosomes, Human, Pair 3;
Chromosomes, Human, Pair 8;
Core Binding Factor Alpha 2 Subunit/genetics;
Female;
Fusion Proteins, bcr-abl/genetics;
Gene Rearrangement;
Humans;
In Situ Hybridization, Fluorescence;
Karyotyping;
Leukemia/diagnosis/*genetics;
Leukemia, Myeloid, Acute/diagnosis/genetics;
Male;
Middle Aged;
Myeloid-Lymphoid Leukemia Protein/genetics;
Oncogene Proteins, Fusion/genetics;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:Journal of Korean Medical Science
2011;26(7):886-892
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the spectrum of chromosomal abnormalities in 325 leukemia patients and developed optimal profiles of leukemic fusion genes for multiplex RT-PCR. We prospectively analyzed blood and bone marrow specimens of patients with acute leukemia. Twenty types of chromosomal abnormalities were detected in 42% from all patients by commercially available multiplex RT-PCR for detecting 28 fusion genes and in 35% by cytogenetic analysis including FISH analysis. The most common cytogenetic aberrations in acute myeloid leukemia patients was PML/PARA, followed by AML1/MGT8 and MLL1, and in acute lymphoid leukemia patients was BCR/ABL, followed by TEL/AML1 and MLL1 gene rearrangement. Among the negative results for multiplex RT-PCR, clinically significant t(3;3)(q21;q26.2), t(8;14)(q24;q32) and i(17)(q10) were detected by conventional cytogenetics. The spectrum and frequency of chromosomal abnormalities in our leukemia patients are differed from previous studies, and may offer optimal profiles of leukemic fusion genes for the development of new molecular detection systems.
