A novel method for studying nuclear localization signal-mediated nuclear translocation.
- Author:
Peng DENG
1
;
Xiaowei GONG
;
Yong JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Active Transport, Cell Nucleus; Cell Nucleus; metabolism; Cholic Acids; Cytological Techniques; HeLa Cells; Humans; Interferon-gamma; metabolism; Nuclear Localization Signals; metabolism; STAT1 Transcription Factor; metabolism; Signal Transduction
- From: Journal of Southern Medical University 2012;32(8):1148-1150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new method for studying the mechanism of nuclear localization signal (NLS)-mediated nuclear translocation in living cells.
METHODSThe cells were treated with 67 mg/L 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS), followed by incubation with 1 g/L wheat germ agglutinin (WGA), and their effects on interferon- γ (IFN-γ)-induced nuclear translocation of signal transducer and activator of transcription 1 (STAT1) were observed.
RESULTSTreatment with CHAPS alone had no effect on IFN-γ-induced nuclear translocation of STAT1, while this process was blocked by further WGA incubation.
CONCLUSIONWe established a new, simple but effective method for studying the mechanism of NLS-mediated nuclear translocation in living cells by perforating the cell membrane with CHAPS treatment.
