Effect of hydrogen inhalation on p38 MAPK activation in rats with lipopolysaccharide- induced acute lung injury.
- Author:
Canxin LIANG
1
;
Xinwei LIU
;
Ling LIU
;
Dongwei HE
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; blood; chemically induced; metabolism; Administration, Inhalation; Animals; Hydrogen; administration & dosage; Lipopolysaccharides; Lung; metabolism; Male; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; blood; metabolism; p38 Mitogen-Activated Protein Kinases; blood; metabolism
- From: Journal of Southern Medical University 2012;32(8):1211-1213
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hydrogen inhalation on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanisms.
METHODSThirty-two male SD rats were randomly divided into 4 groups (n=8), namely the normal saline group (SA), saline with 2% hydrogen gas inhalation group (SH group), ALI group, and ALI with hydrogen inhalation group (LH group). In the two ALI groups, ALI was induced by intraperitoneal injection of 15 mg/kg LPS. Treatments with inhalation of 2% hydrogen gas for 6 h was administered after the injection of LPS or saline. The concentrations of tumor necrosis factor-α (TNF-α) in the lung tissue and serum were examined with ELISA. The expression of p38 MAPK in the lung tissue was detected by Western blotting..
RESULTSHydrogen inhalation decreased the expression of p-p38 MAPK in the lung tissue, and significantly reduced TNF-α content in the lung tissue and serum of rats with ALI.
CONCLUSIONHydrogen inhalation can decrease the expression of TNF-α in the lung tissue and serum, and this effect may be related with reduced p38 MAPK expression and inhibition of p38 MAPK activation.
