Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts.
- Author:
Chao YUAN
1
;
Mingyan XU
;
Gengzhen CHEN
;
Yucai FU
;
Xiaoling DENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cells, Cultured; Chemokine CCL2; metabolism; Fibroblasts; metabolism; Humans; Indoles; pharmacology; Lung; cytology; metabolism; Protein Kinase C; antagonists & inhibitors; Thrombin; pharmacology
- From: Journal of Southern Medical University 2012;32(9):1250-1254
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1).
METHODSCultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR.
RESULTSThe broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects.
CONCLUSIONCa(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.
