Magnetic-activated cell sorting combined with multiplex ligation-dependent probe amplification for detecting molecular cytogenetic abnormalities in multiple myeloma.
- Author:
Ming LEI
1
;
Li MA
;
Liping KUANG
;
Yan PANG
;
Jiulong WU
;
Zhifang XIAO
;
Li LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chromosome Aberrations; Female; Humans; Immunomagnetic Separation; In Situ Hybridization, Fluorescence; Karyotyping; Male; Middle Aged; Multiple Myeloma; diagnosis; genetics; Multiplex Polymerase Chain Reaction
- From: Journal of Southern Medical University 2012;32(9):1332-1335
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the reliability of magnetic-activated cell sorting (MACS) combined with multiplex ligation-dependent probe amplification (MLPA) in the detection of the molecular cytogenetic abnormalities in multiple myeloma.
METHODSThe bone marrow cells were collected from 29 patients with multiple myeloma. The immuno magnetically sorted and unsorted cells were detected for TP53 and RB1 expressions using MLPA probes and the results were compared with fluorescent in situ hybridization (FISH).
RESULTSThe detection success rate was 100% for MLPA, which yielded results with an concordance rate of 99.1% with the FISH results. The positivity rates of MLPA and FISH were both increased after immunomagnetic sorting of the bone marrow cells.
CONCLUSIONMLPA can well suit the clinical needs for detecting molecular cytogenetic abnormalities in multiple myeloma, and the samples should be immuno magnetically sorted before the assay.
