Effect of docetaxel on C-jun and androgen receptor interaction in prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells.
- Author:
Binshen CHEN
1
;
Yawen XU
;
Abai XU
;
Chunxiao LIU
;
Shaobo ZHENG
;
Hulin LI
;
Kai XU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Male; Prostate; metabolism; Prostatic Neoplasms; metabolism; Proto-Oncogene Proteins c-jun; metabolism; Receptors, Androgen; metabolism; Taxoids; pharmacology
- From: Journal of Southern Medical University 2012;32(10):1461-1464
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of docetaxel on the interaction between C-jun and androgen receptor (AR) in prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells.
METHODSLNCaP and LNCaP-bic cells were treated with docetaxel and the changes in AR and AP-1 expression were evaluated using luciferase assay. Western blotting and immunoprecipitation assay were employed to analyze the effects of docetaxel on the expressions of C-jun and AR and their interaction.
RESULTSLuciferase assay showed that LNCaP and LNCaP-bic cells expressed higher levels of AR and C-jun after docetaxel treatment. Docetaxel induced a higher level of p-c-jun expression in LNCaP-bic cells than in the parental LNCaP cells. Western blotting showed a strong PSA protein expression in LNCaP-bic cell after docetaxel treatment. Docetaxel caused a stronger interaction between AR and C-jun in LNCaP-bic cells.
CONCLUSIONDocetaxel activates ligand-independent AR transcription, and the interaction between AR and C-jun may affect the outcome of docetaxel chemotherapy.
