Effect of Panax notoginseng on genes expression of CYP and GST in liver tissues of rats.
- Author:
Xiufen YANG
1
;
Meichun LIAO
;
Ziming YANG
;
Jingjing GUO
;
Qianqian GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytochrome P-450 Enzyme System; genetics; metabolism; Drugs, Chinese Herbal; pharmacology; Gene Expression; drug effects; Glutathione Transferase; genetics; metabolism; Liver; drug effects; enzymology; Male; Panax notoginseng; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2009;34(18):2390-2393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the effects of Panax notoginseng on the expression of cytochrome P450 (CYP) genes and glutathione S-trans-ferase (GST) genes in liver tissues of male SD rats.
METHODRats were administered P. notoginseng 2 or 4 g x kg(-1) bw/d by gavage daily for 14 days. The levels of gene expression of CYP1A1, CYP1A2, CYP2B1, CYP2E1, CYP3A1, CYP4A1, and GSTml, GST-pi were examined by quantitative real-time reverse-transcription polymerase chain reaction (quantitative real time-RT-PCR) assays.
RESULTThe expression of CYP1A1, CYP1A2, CYP2E1, CYP3A1, GSTml and GST-pi genes was not changed by 2 or 4 g x kg(-1) P. notoginseng treatment, But P. notoginseng significantly inhibited mRNA expressions of CYP2B1 (0.48-fold, P < 0.05, and 0.61-fold, P < 0.05, respectively) and CYP 4A1 (0.69-fold, and 0. 51-fold, respectively).
CONCLUSIONP. notoginseng had a special inhibitory selectivity on the expression of CYP2B1 and CYP4A1 genes in liver tissues of rats, which indicated it may be one of the mechanisms of actions of P. notoginseng. P. notoginseng had no effects on the expressions of CYP1A1, CYP1A2, CYP2E1 and CYP3A1 genes, which suggested when P. notoginseng co-administrated with those drugs metabolized by the above major metabolizing enzymes in liver, metabolic herb-drug interactions would not be happened.
