Establishment of transgenic receptor system and aFGF transformation in astragalus.
- Author:
Dachao LI
1
;
Yi WANG
;
Shicui JIANG
;
Xiuwen HAN
;
Meiping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus Plant; genetics; metabolism; Fibroblast Growth Factor 1; genetics; metabolism; Gene Expression Regulation; Genetic Engineering; methods; Humans; Plants, Genetically Modified; genetics; metabolism; Transformation, Genetic
- From: China Journal of Chinese Materia Medica 2009;34(19):2454-2457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a high-frequency regeneration system of Astragalus and an aFGF transformation system.
METHODCotyledon node of the Astragalus explants was used for organogenesis to establish a high-frequency regeneration system. GV3101 was used to transform cotyledon node, and aFGF gene was introduced into Astragalus, renewable strain was detected by PCR.
RESULTAll cotyledon node was explants, adventitious buds were induced in the medium of MS +2.0 mg x L(-1) BA +0.5 mg x L(-1) IBA, the root was taken in the medium of 1/2MS +5.0 mg x L(-1) NAA to give a high frequency regeneration system. All cotyledon node was precultured in medium for 3 days and infected with Agrobacterium (A600 0.3) for 10 min and then cocultured for 2 days. The aFGF gene was confirmed to transform into genome of Astragalus.
CONCLUSIONA high-frequency regeneration system of Astragalus and an aFGF transformation system were established.
